Journal: Bioactive Materials

Article Title: Glucose/ROS-responsive and redox-gated adaptive hydrogel dressing for accelerating diabetic wound repair via synergistic cGAS/STING pathway inhibition and oxidative stress alleviation

doi: 10.1016/j.bioactmat.2026.03.025

Figure Lengend Snippet: Angiogenesis and collagen deposition in diabetic wound tissues following HPSL@SG hydrogel treatment. (A) Dihydroethidium (DHE) immunofluorescence staining and (B) semi-quantitative analysis of wound tissues from each treatment group on day 7, scale bar = 100 μm. Immunofluorescence staining of (C) MMP-9, IL-6, and IL-10, and (D) CD31, VEGF-A, and collagen I in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. (E-J) Mean relative fluorescence intensity of each indicator in wound tissue sections from each treatment group on day 7, scale bar = 100 μm. All data are shown as mean ± SEM (n = 6).

Article Snippet: IL-6 and IL-10-specific antibodies were purchased from Bosterbio (Wuhan, China).

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: bioRxiv

Article Title: High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain

doi: 10.64898/2026.05.29.728695

Figure Lengend Snippet: a , Schematic illustration of HLNP-mediated mRNA delivery and translation. b-d , Luciferase activity of HLNP treated N2a neuronal cells (b, Samples 56 and 110 had n = 3; all other groups had n = 4), BV2 microglial cells (c, Samples 52, 56, 58, 60 and 69 had n = 3; all other groups had n = 4) and bEnd.3 brain endothelial cells (d, n = 4). e , Representative fluorescence images of N2a and BV2 cells following delivery of mCherry mRNA by selected HLNP formulations. Blank indicates untreated cells. Scale bar, 100 μm. f , Schematic illustration of HLNP-mediated siRNA delivery. g , Relative Ppib mRNA levels in N2a cells following siRNA delivery, quantified by RT-qPCR and normalized to housekeeping gene Gapdh ( n = 4). h, Representative ex vivo bioluminescence images of isolated brains collected from mice following intravenous administration of luciferase mRNA-loaded LNPs (51, 72, and Pati) (top). Corresponding ex vivo images of major organs (bottom) show distinct biodistribution patterns among the formulations. i , Quantification of normalized luminescence intensity in the brain and liver based on the ex vivo imaging shown in panel h ( n = 3).

Article Snippet: Ppib mouse pre-designed siRNA Set was purchased from MedChem Express.

Techniques: Luciferase, Activity Assay, Fluorescence, Quantitative RT-PCR, Ex Vivo, Isolation, Imaging

Journal: bioRxiv

Article Title: High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain

doi: 10.64898/2026.05.29.728695

Figure Lengend Snippet: a , Schematic illustration of the in vivo DNA-barcoded HLNP screening workflow. Mice were intravenously administered pooled HLNP libraries containing uniquely barcoded formulations. Following systemic circulation, brains were harvested and dissociated into single-cell suspensions, and major brain cell populations were isolated. HLNP-associated DNA barcodes were recovered from each cell population and quantified by NGS to identify formulations enriched in specific brain cell types. b , Heatmap showing normalized barcode enrichment of individual HLNP formulations across major brain cell populations, including neurons, microglia, astrocytes, endothelial cells and oligodendrocytes. Color intensity represents the relative enrichment of each formulation within the indicated cell type. Values were independently normalized within each cell type. c , Schematic illustration of neuron-targeted delivery of Pten siRNA by HLNPs and subsequent activation of the PI3K–AKT signaling pathway to promote axonal growth. d , Flow cytometric analysis of PTEN protein expression in N2a cells following treatment with HLNP 69 encapsulating Pten siRNA. Data are presented as mean fluorescence intensity (MFI) ( n = 4). e , Representative confocal images of N2a cells stained for nuclei (blue), F-actin (green), and PTEN (red) following treatment with HLNP 69–encapsulated Pten siRNA. Scale bar, 50 μm. f , Experimental timeline for TBI induction, intravenous administration of HLNP– Pten siRNA formulations, behavioral assessment, and downstream molecular and histological analyses. g , Relative Pten mRNA expression in brain tissue measured by RT–qPCR following treatment with HLNP 69–encapsulated Pten siRNA ( n = 3). h , Neurological function assessed using the mNSS at 29 days post-injury ( n = 3 for Untreated, n = 4 for TBI, n = 5 for Control siRNA and Pten siRNA). i , Representative immunofluorescence images of brain sections stained for NeuN and GFAP. Scale bar, 300 μm. j , Quantification of NeuN and GFAP fluorescence intensity in brain sections ( n = 3). Data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA with appropriate post hoc multiple-comparison tests.

Article Snippet: Ppib mouse pre-designed siRNA Set was purchased from MedChem Express.

Techniques: In Vivo, Single Cell, Isolation, Formulation, Activation Assay, Expressing, Fluorescence, Staining, Quantitative RT-PCR, Control, Immunofluorescence, Comparison

Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Article Title: Characterization of HIV Humoral Immunity during Analytical Treatment Interruption

doi: 10.1016/j.jcv.2026.105930

Figure Lengend Snippet: Longitudinal serology in 3 representative participants (acute and chronic treated, post-treatment controller), including 5 different assays. ATI duration is shown with gray shading, followed by ART re-initiation. (HIVc= Ortho Vitros HIV Combo; aHIV= Ortho Vitros HIV 1+2; LAg= Sedia Limiting Antigen avidity assay; Ag= BioRad BioPlex 2200 p24 antigen; M Ab= BioRad BioPlex 2200 HIV group M Ab).

Article Snippet: ATI duration is shown with gray shading, followed by ART re-initiation. (HIVc= Ortho Vitros HIV Combo; aHIV= Ortho Vitros HIV 1+2; LAg= Sedia Limiting Antigen avidity assay; Ag= BioRad BioPlex 2200 p24 antigen; M Ab= BioRad BioPlex 2200 HIV group M Ab).

Techniques: